ABSTRACT
We conducted a molecular epidemiological study to investigate HIV-1 strains in Rio Grande, southern Brazil, searching for an association with transmission mode and risk behavior. Patients (185) identified at an AIDS treatment reference Hospital, from 1994 to 1997, were included; from which 107 blood samples were obtained. Nested PCR was realized once for each sample; for amplified samples (69) HIV subtypes were classified using the heteroduplex mobility assay. Subtypes identified were B (75 percent), C (22 percent) and F (3 percent). All infections with C were diagnosed after 1994. Comparing patients with B and C, no differences were detected regarding demographic, clinical and laboratory characteristics; survival analysis did not reveal differences in HIV to AIDS evolution. A higher proportion of injecting drug users, IDU (not significant, p<.07) was found among those with C. This suggests that C may have been introduced in this area through IDU, and is being spread, probably by their sexual partners, to persons with other risk practices
Subject(s)
Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Acquired Immunodeficiency Syndrome/epidemiology , DNA, Viral/genetics , HIV-1 , Acquired Immunodeficiency Syndrome/transmission , Brazil/epidemiology , Genetic Variation , Heteroduplex Analysis , HIV-1 , Molecular Epidemiology , Polymerase Chain Reaction , Risk FactorsABSTRACT
We report results of nucleotide sequencing and phylogenetic analysis of the env gene of 11 HIV-1 isolates, in Belo Horizonte, Brazil. Ten isolates belonged to HIV-1 subtype B and one was a probable B/F mosaic. This putative B/F recombinant is similar but not identical in its nucleotide sequence to other B/F mosaics described in Brazil.
Subject(s)
Genetic Variation , HIV-1/genetics , Polymerase Chain Reaction , HIV-1/isolation & purification , Genome , Base Sequence/genetics , Acquired Immunodeficiency Syndrome/geneticsABSTRACT
A freqüência de rotavírus do grupo A foi avaliada em 194 amostras de fezes de bezerros, com idade entre 2 e 60 dias, provenientes de 33 propriedades da bacia leiteira de Pará de Minas, Minas Gerais. Utilizando um sistema de ELISA monoclonal direto, 33 (17 porcento)destas amostras foram positivas para rotavírus do grupo A. Do total de animais positivos para rotavírus do grupo A, 60,6 porcento apresentavem diarréia no momento da coleta. Das 33 propriedades amostradas, a presença de rotavírus foi detectada em 19 (57,6 porcento). Dentre todas as amostras examinadas, somente 7 apresentaram simultaneamente rotavírus e Cryptosporidium spp